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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
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Related Experiment Video

Updated: Aug 5, 2025

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
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Sequence-specific DNA labelling for fluorescence microscopy.

Shalini Pradhan1, Sinem Apaydin1, Jonas Bucevičius1

  • 1Chromatin Labelling and Imaging Group, Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Am Fassberg 11, 37077, Göttingen, Germany.

Biosensors & Bioelectronics
|March 29, 2023
PubMed
Summary

New DNA labeling methods preserve nucleus structure for advanced cell imaging. These techniques offer less invasive alternatives to traditional methods, enabling live-cell chromatin studies.

Keywords:
Chromatin imagingDNA methyltransferasesPolyamidesTranscription activator-like effectorsTriplex forming oligonucleotidesdCas9

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genomics

Background:

  • Preserving nucleus structure is crucial for understanding genome organization and gene regulation.
  • Traditional DNA imaging methods can be harsh and may alter cellular structures.

Purpose of the Study:

  • To review sequence-specific DNA labeling methods for microscopy.
  • To highlight non-denaturing techniques compatible with live-cell imaging.

Main Methods:

  • Hairpin polyamides
  • Triplex-forming oligonucleotides
  • dCas9 proteins
  • Transcription activator-like effectors (TALEs)
  • DNA methyltransferases (MTases)

Main Results:

  • These methods allow imaging in fixed and/or living cells without harsh treatment.
  • Repetitive DNA loci and specific regions like telomeres/centromeres can be identified.
  • Visualizing single-copy sequences remains a challenge.

Conclusions:

  • Less invasive DNA labeling methods are emerging as alternatives to fluorescence in situ hybridization (FISH).
  • These advanced techniques, combined with super-resolution microscopy, promise to reveal unperturbed chromatin structure and dynamics in living systems.