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LINC01116 modulates EMT process via binding with AGO1 mRNA in oesophageal squamous cell carcinoma

Xin Fang1, Li-Hua Ren2, Sachin Mulmi Shrestha1

  • 1Medical College, Southeast University, Nanjing 210009, Jiangsu Province, China.

Biochimica Et Biophysica Acta. Molecular Cell Research
|March 29, 2023
PubMed

Insights

Long non-coding RNA LINC01116 promotes esophageal squamous cell carcinoma (OSCC) progression and metastasis. It activates AGO1 expression, driving the epithelial-mesenchymal transition (EMT) process in OSCC cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in various diseases.
  • The specific roles and mechanisms of antisense lncRNAs in esophageal squamous cell carcinoma (OSCC) are not fully understood.

Purpose of the Study:

  • To investigate the role and molecular mechanism of LINC01116 in the development and progression of OSCC.

Main Methods:

  • Analysis of RNA sequencing data and online databases.
  • Validation of LINC01116 expression in OSCC and intraepithelial neoplasia (IEN) specimens.
  • In vitro and in vivo functional assays to assess LINC01116's impact on OSCC progression and metastasis.
  • Investigation of the molecular mechanism involving AGO1 activation and epithelial-mesenchymal transition (EMT).

Main Results:

  • LINC01116 was found to be upregulated in OSCC and IEN tissues.
  • Overexpression of LINC01116 promoted OSCC cell proliferation, migration, and invasion in vitro and tumor growth and metastasis in vivo.
  • LINC01116, predominantly expressed in OSCC cells (cytoplasmic), activates AGO1 expression by binding to AGO1 mRNA.
  • This activation facilitates the EMT process, contributing to OSCC progression.

Conclusions:

  • LINC01116 is an oncogenic lncRNA that promotes OSCC progression and metastasis.
  • The mechanism involves LINC01116-induced AGO1 activation and subsequent EMT.
  • LINC01116 may serve as a potential diagnostic biomarker and therapeutic target for OSCC.
Keywords:
AGO1EMTLINC01116Oesophageal squamous cell carcinoma

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