Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hypoxia01:23

Hypoxia

1.1K
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
1.1K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Heterochromatin02:38

Heterochromatin

14.0K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
14.0K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

5.9K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.9K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systematic characterisation of site-specific proline hydroxylation using hydrophilic interaction chromatography and mass spectrometry.

eLife·2026
Same author

PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progression.

eLife·2026
Same author

Exploring hypoxia-related genes as prognostic indicators in lung adenocarcinoma.

PloS one·2026
Same author

Mechanisms controlling transcription in hypoxia; an unexpected role of NF-κB.

Free radical biology & medicine·2026
Same author

Imaging vascular characteristics and glycolytic metabolism of glioblastoma in a chick embryo model using <sup>1</sup>H MRI and [<sup>18</sup>F]FDG-PET.

Molecular imaging and biology·2026
Same author

Rethinking the role of HIF in hepatocellular carcinoma.

The FEBS journal·2026

Related Experiment Video

Updated: Aug 5, 2025

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

18.2K

Functional crosstalk between chromatin and hypoxia signalling.

Harry Collier1, Adam Albanese1, Chun-Sui Kwok1

  • 1Institute of Systems Molecular and Integrative Biology, University of Liverpool, United Kingdom.

Cellular Signalling
|March 29, 2023
PubMed
Summary

Chromatin structure regulates gene expression and cellular responses to low oxygen (hypoxia). This review explores how hypoxia influences chromatin, focusing on histone modifications and hypoxia-inducible factors.

Keywords:
AcetylationChromatinChromatin remodellersHATHDACHIFHMTHypoxiaKDMMethylation

More Related Videos

Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

85.1K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K

Related Experiment Videos

Last Updated: Aug 5, 2025

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

18.2K
Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

85.1K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

6.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic genomes are organized into chromatin, a complex of DNA and histone proteins.
  • Chromatin plays a crucial role in regulating gene expression by controlling DNA accessibility.
  • Sensing and responding to hypoxia is vital in physiological and pathological processes.

Purpose of the Study:

  • To review the mechanisms controlling chromatin structure under hypoxic conditions.
  • To highlight the interplay between chromatin regulators and hypoxia-inducible factors.
  • To identify existing knowledge gaps in the field.

Main Methods:

  • Literature review focusing on recent findings in hypoxia and chromatin regulation.
  • Analysis of mechanisms involving histone modifications and chromatin remodelers.
  • Integration of data on hypoxia-inducible factors and their interaction with chromatin.

Main Results:

  • Hypoxia intricately influences chromatin structure and function.
  • Histone modifications and chromatin remodelers are key players in hypoxia-mediated gene expression changes.
  • Hypoxia-inducible factors are integrated with chromatin regulatory pathways.

Conclusions:

  • The link between oxygen levels and chromatin regulation is a critical area of study.
  • Further research is needed to fully elucidate the complex interactions between chromatin and hypoxia.
  • Understanding these mechanisms has implications for both normal physiology and disease states.