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Related Experiment Videos

DNA compaction during intense transcription measured by electron microscope tomography.

A L Olins, D E Olins, H A Levy

    European Journal of Cell Biology
    |March 1, 1986
    PubMed
    Summary

    Electron microscope tomography revealed that hyperstimulated Balbiani ring genes in Chironomus tentans exhibit a low DNA compaction ratio. This suggests significant nucleosome unfolding during active gene transcription.

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    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Genetics

    Background:

    • Balbiani rings (BR) are large transcription sites in insect salivary glands.
    • Understanding gene structure and DNA organization is crucial for gene regulation studies.

    Purpose of the Study:

    • To investigate the in situ DNA compaction ratio of hyperstimulated Balbiani ring genes in Chironomus tentans.
    • To estimate the degree of nucleosome unfolding in actively transcribing genes.

    Main Methods:

    • Electron microscope tomography (EMT) was used to reconstruct thick sections of Chironomus tentans salivary glands.
    • Six transcription loops of pilocarpine-hyperstimulated BR genes were analyzed at low resolution.
    • Transcription unit length was measured in situ and compared to coding region DNA length.

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    Main Results:

    • The average transcription unit length of BR genes was measured.
    • The in situ DNA compaction ratio was estimated to be approximately 3-fold.
    • This low compaction ratio indicates significant nucleosome unfolding.

    Conclusions:

    • Hyperstimulated Balbiani ring genes in Chironomus tentans exhibit a low DNA compaction ratio.
    • The findings suggest a substantial degree of nucleosome unfolding in these actively transcribing genes.
    • This provides insights into the structural organization of highly active genes.