Development of ZnO/selenium nanoparticles embedded chitosan-based anti-bacterial wound dressing for potential healing...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Regenerative Medicine

Background:

• Infected wound healing in pediatric surgery presents challenges.
• Advanced biomaterials are needed to improve treatment outcomes.
• Chitosan-based scaffolds offer potential for wound care applications.

Purpose of the Study:

• To assess the efficacy of a chitosan-ZnO/Selenium nanoparticle scaffold for infected wound healing in pediatric surgery.
• To investigate the structural, chemical, and morphological properties of the synthesized nanoparticles.
• To evaluate the antibacterial, biocompatibility, and in-vivo wound healing capabilities of the scaffold.

Main Methods:

• Nanoparticle scaffolds synthesized using chitosan (CS), zinc oxide (ZnO), and Selenium (SeNPs) via freeze-drying.
• Characterization using UV-Vis, FTIR, X-ray diffraction, and Scanning Electron Microscopy.
• In-vitro studies with NIH 3T3 and HaCaT cell lines; in-vivo assessment of wound healing in a pediatric fracture surgery model.

Main Results:

• The chitosan-ZnO/SeNPs scaffold demonstrated significant antioxidant and antimicrobial activity against E. coli and S. aureus.
• In-vitro studies confirmed excellent biocompatibility, cell adhesion, viability, and proliferation.
• In-vivo studies showed enhanced collagen synthesis, re-epithelialization, and accelerated wound closure with improved histopathological indices.

Conclusions:

• The synthesized chitosan-ZnO/SeNPs nanoparticle scaffold is a promising therapeutic agent for infected wound healing in pediatric surgery.
• The scaffold's combined properties of antimicrobial activity and biocompatibility promote rapid and effective tissue regeneration.
• This novel scaffold significantly improves wound healing outcomes, offering a potential advancement in pediatric surgical care.