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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
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Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Related Experiment Video

Updated: Aug 5, 2025

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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Continuous Multiplexed Phage Genome Editing Using Recombitrons.

Chloe B Fishman1, Kate D Crawford1,2, Santi Bhattarai-Kline1,3

  • 1Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA.

Biorxiv : the Preprint Server for Biology
|March 30, 2023
PubMed
Summary
This summary is machine-generated.

Scientists developed recombitrons, a new method for engineering bacteriophage (phage) genomes. This scalable technology enables efficient and continuous modification of phage DNA, accelerating phage-based solutions for eliminating harmful bacteria.

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Related Experiment Videos

Last Updated: Aug 5, 2025

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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The MultiBac Protein Complex Production Platform at the EMBL
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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Bacteriophages (phages) are natural regulators of bacterial communities and hold potential as biological agents against pathogens.
  • Phage genome editing is crucial for enhancing phage efficacy but has been hampered by low efficiency and laborious methods.
  • Existing techniques limit the scope and speed of phage modification, hindering innovation in phage-based technologies.

Conclusions:

  • Recombitrons offer a significant advancement in phage genome engineering, providing a scalable and efficient approach.
  • This technology facilitates rapid development of improved phage-based solutions for controlling pathogenic bacteria.
  • The method accelerates research and innovation in the field of phage therapy and biocontrol.