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Alamar Blue assay optimization to minimize drug interference and inter-assay viability
Mina N Dinh1, Masahiro Hitomi1, Zahraa A Al-Turaihi1
1Department of Translational Hematology & Oncology Research, Cleveland Clinic, Cleveland, OH.
Biorxiv : the Preprint Server for Biology
|March 30, 2023
Summary
A modified Alamar Blue assay accurately measures EGFR inhibitors in non-small cell lung cancer cells. Removing drug-containing medium before testing prevents false readings, ensuring reliable cell viability results.
Area of Science:
- Cell Biology
- Pharmacology
- Biochemistry
Background:
- Alamar Blue (AB) is a popular, cost-effective, and nondestructive reagent for cell viability assays.
- Unexpected right-shifts in dose-response curves were observed when evaluating osimertinib, an EGFR inhibitor, on PC-9 cells using AB compared to Cell Titer Glo.
- Previous studies noted that some redox drugs directly interfere with AB readings, but osimertinib did not directly increase AB readings.
Conclusions:
- The modified Alamar Blue assay provides an accurate method for assessing the efficacy of EGFR targeted therapies.
- This improved assay overcomes limitations of previous methods, ensuring reliable cell viability measurements.
- The method is suitable for drug screening and longitudinal studies in cancer research.
