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Related Experiment Video

Updated: Aug 4, 2025

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Terahertz time-domain attenuated total reflection spectroscopy integrated with a microfluidic chip.

Ying Fu1,2, Tunan Chen3, Ligang Chen1,2

  • 1Center of Super-Resolution Optics & Chongqing Engineering Research Center of High-Resolution and Three-Dimensional Dynamic Imaging Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, China.

Frontiers in Bioengineering and Biotechnology
|March 30, 2023
PubMed
Summary

We developed a polydimethylsiloxane microfluidic chip for terahertz time-domain attenuated total reflection (THz TD-ATR) spectroscopy. This chip enables accurate aqueous sample analysis by determining optimal cavity depth for spectral data interpretation.

Keywords:
attenuated total reflectionevanescent fieldlactate dehydrogenasemicrofluidic chipterahertz

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Area of Science:

  • Spectroscopy
  • Microfluidics
  • THz technology

Background:

  • Accurate measurement of aqueous samples using terahertz time-domain attenuated total reflection (THz TD-ATR) spectroscopy is crucial.
  • Limited research exists on integrating microfluidic chips for this purpose.

Purpose of the Study:

  • To fabricate a polydimethylsiloxane microfluidic chip (M-chip) for aqueous sample analysis.
  • To investigate the impact of M-chip cavity depth on THz spectra.
  • To establish guidelines for analyzing THz TD-ATR spectral data based on M-chip configuration.

Main Methods:

  • Fabrication of a polydimethylsiloxane microfluidic chip.
  • THz TD-ATR spectroscopy measurements of pure water, physiological solution, and protein solution.
  • Analysis of THz spectral data using Fresnel formulae for one- and two-interface models.

Main Results:

  • The choice of Fresnel formula model (one- or two-interface) for THz spectral data analysis depends on the M-chip cavity depth.
  • A cavity depth < 210 μm requires the two-interface model.
  • A cavity depth ≥ 210 μm allows the use of the one-interface model.

Conclusions:

  • This study provides a method for optimizing microfluidic chip design for THz TD-ATR spectroscopy.
  • The findings facilitate accurate analysis of aqueous biological samples using THz TD-ATR.
  • This work promotes the application of THz TD-ATR spectroscopy in biological and chemical research.