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Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins
Lara Hauke1, Sebastian Isbaner1, Arindam Ghosh1
1Third Institute of Physics - Biophysics, Georg August University, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany.
ACS Nano
|March 30, 2023
Summary
Metal-induced energy transfer (MIET) imaging now enables super-resolution microscopy for live cells using fluorescent proteins. This technique provides nanometer axial mapping of cellular components with minimal phototoxicity.
Area of Science:
- Biophysics
- Cell Biology
- Super-resolution Microscopy
Background:
- Metal-induced energy transfer (MIET) imaging offers super-resolution axial localization.
- Current limitations exist for live-cell applications with fluorescent proteins.
Purpose of the Study:
- To demonstrate the applicability and capabilities of MIET imaging for live-cell imaging using fluorescent proteins.
- To assess MIET imaging's performance across diverse cell types and fluorescent proteins.
Main Methods:
- Implementation of MIET imaging for live-cell microscopy.
- Utilized various cell types (human stem cells, osteosarcoma cells, Dictyostelium discoideum).
- Employed diverse fluorescent proteins (GFP, mScarlet, RFP, YPet).
Main Results:
- Achieved nanometer axial mapping of living cellular and subcellular structures.
- Demonstrated applicability across multiple cell types and fluorescent proteins.
- Showcased imaging capabilities over time scales from milliseconds to hours with negligible phototoxicity.
Conclusions:
- MIET imaging is a viable super-resolution technique for live-cell studies with fluorescent proteins.
- The method provides high-resolution axial information with minimal phototoxic effects.
- Broad applicability in live-cell biophysical and biological research.
