Delayed Outgrowth in Response to the BDNF and Altered Synaptic Proteins in Neurons From SHR Rats
Daniela M Marques1, Amanda S Almeida1, Catiane B A Oliveira1
1Departamento de Bioquímica, Programa de Pós-Graduação Em Ciências Biológicas-Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-anexo, Porto Alegre, RS, 90035-003, Brasil.
Neurochemical Research
|March 30, 2023
Summary
Neurons from an ADHD rat model show delayed development and reduced branching. These cells also exhibit altered protein levels and a diminished response to BDNF, offering insights into synaptic dysfunction and potential ADHD treatments.
Area of Science:
- Neuroscience
- Developmental Biology
- Genetics
Background:
- Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental disorder with known alterations in brain development.
- Previous research indicates delayed cortical and subcortical development in children with ADHD.
Purpose of the Study:
- To investigate the in vitro development of frontal cortical neurons from an ADHD rat model (SHR) compared to controls (WKY).
- To assess the impact of BDNF treatment on these neurons and evaluate synaptic protein levels.
Main Methods:
- Cultured frontal cortical neurons from SHR and WKY rats were studied over time in vitro.
- Neurons were treated with BDNF at different developmental time points (DIV).
- Analysis included dendritic morphology, synaptic protein levels, and BDNF-related proteins.
Main Results:
- SHR neurons displayed reduced dendritic outgrowth and branching compared to WKY neurons.
- CREB levels decreased at 1 DIV, and SNAP-25 decreased at 5 DIV in SHR neurons.
- Exogenous BDNF treatment resulted in less dendritic branching in SHR neurons, unlike in WKY neurons.
Conclusions:
- ADHD model neurons exhibit developmental delays and altered synaptic protein expression.
- These neurons show a reduced response to BDNF, potentially linked to early transcription factor changes.
- This in vitro model offers a tool for studying ADHD-related synaptic dysfunction and evaluating therapeutic interventions.
