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Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice
Published on: November 3, 2013
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Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique.
Katsumasa Goto1, Kazuya Ohashi2
1Department of Physiology, Graduate School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan. gotok@sepia.ocn.ne.jp.
Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2023
Summary
This study details nerve transection methods in mice for skeletal muscle atrophy research. Understanding denervation techniques is crucial for studying muscle plasticity and neurotrophic factors.
Area of Science:
- Neuroscience
- Muscle Physiology
- Skeletal Muscle Research
Background:
- Nerve transection is a validated model for skeletal muscle atrophy.
- Mouse models are increasingly used due to genetic tools like transgenic and knockout strains.
- Sciatic and tibial nerve denervation are common procedures in rodent models.
Purpose of the Study:
- To demonstrate and explain sciatic and tibial nerve transection procedures in mice.
- To facilitate research on skeletal muscle plasticity.
- To advance understanding of neurotrophic factors' role in muscle physiology.
Main Methods:
- Detailed surgical procedures for sciatic nerve transection in mice.
- Detailed surgical procedures for tibial nerve transection in mice.
- Focus on techniques applicable to common rodent models.
Main Results:
- Provides a clear, step-by-step guide for nerve transection in mice.
- Highlights the accessibility of sciatic and tibial nerve resection.
- Establishes a reproducible methodology for skeletal muscle denervation studies.
Conclusions:
- The described methods enable reliable skeletal muscle denervation in mice.
- Facilitates further investigation into nerve-muscle interactions and atrophy.
- Supports the use of mouse models for studying muscle plasticity and neurotrophic factor roles.
