Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Yap1 regulates motility and vertebral development and prevents kyphoscoliosis in zebrafish.

PLoS genetics·2026
Same author

Restorative macrophage-derived RNAseT2 stimulates muscle stem cell fusion via an SLK/N-WASP/actin bundling dependent axis.

Nature communications·2026
Same author

Open Raman Microscopy (ORM): A Modular Hardware and Software Framework for Accessible Raman Imaging.

bioRxiv : the preprint server for biology·2025
Same author

Muscle sparing through differential nutritional control of three muscle growth mechanisms: how zebrafish larvae deal with starvation.

Developmental biology·2025
Same author

Oxidative phosphorylation is required for cardiomyocyte re-differentiation and long-term fish heart regeneration.

Nature cardiovascular research·2025
Same author

Muscle Regeneration Can Be Rescued in a Telomerase Deficient Zebrafish Model of Ageing by MMP Inhibition.

Aging cell·2025

Related Experiment Video

Updated: Aug 4, 2025

Examining Muscle Regeneration in Zebrafish Models of Muscle Disease
07:58

Examining Muscle Regeneration in Zebrafish Models of Muscle Disease

Published on: January 18, 2021

5.1K

Skeletal Muscle Regeneration in Zebrafish.

Tapan G Pipalia1, Sami H A Sultan2, Jana Koth1,3

  • 1Randall Centre for Cell & Molecular Biophysics, King's College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2023
PubMed
Summary

This study introduces simple, effective zebrafish muscle regeneration protocols. These methods allow detailed monitoring of muscle repair in individual larvae, enhancing understanding of stem cell and immune cell roles.

Keywords:
Confocal live imagingLaser injuryMultiphoton microscopyMuscle precursor cellsMuscle regenerationMuscle woundingNeedlestick injuryPax7Satellite cellSecond harmonic generationSpinning disk imagingZebrafish muscle

More Related Videos

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
07:13

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Published on: January 30, 2013

10.5K
Author Spotlight: A Cryoinjury Model for Studying Skeletal Muscle Regeneration of the Caudal Peduncle in Adult Zebrafish
07:29

Author Spotlight: A Cryoinjury Model for Studying Skeletal Muscle Regeneration of the Caudal Peduncle in Adult Zebrafish

Published on: July 7, 2023

2.6K

Related Experiment Videos

Last Updated: Aug 4, 2025

Examining Muscle Regeneration in Zebrafish Models of Muscle Disease
07:58

Examining Muscle Regeneration in Zebrafish Models of Muscle Disease

Published on: January 18, 2021

5.1K
Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
07:13

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

Published on: January 30, 2013

10.5K
Author Spotlight: A Cryoinjury Model for Studying Skeletal Muscle Regeneration of the Caudal Peduncle in Adult Zebrafish
07:29

Author Spotlight: A Cryoinjury Model for Studying Skeletal Muscle Regeneration of the Caudal Peduncle in Adult Zebrafish

Published on: July 7, 2023

2.6K

Area of Science:

  • Developmental Biology
  • Regenerative Medicine
  • Zebrafish Models

Background:

  • Muscle regeneration studies inform therapeutic strategies.
  • Rodent models are established, but zebrafish offer genetic and optical advantages.
  • Existing zebrafish muscle wounding protocols can be improved for precision and adaptability.

Purpose of the Study:

  • To describe simple, cost-effective, and precise protocols for larval zebrafish skeletal muscle regeneration.
  • To establish adaptable methods for analyzing muscle repair over time in individual larvae.
  • To facilitate detailed observation of muscle stem cells, immune cells, and fiber regeneration.

Main Methods:

  • Development of two distinct larval zebrafish skeletal muscle wounding protocols (chemical and physical).
  • Establishment of analysis methods for monitoring muscle damage, stem cell and immune cell infiltration, and fiber regeneration.
  • Longitudinal observation of regeneration processes within individual zebrafish larvae.

Main Results:

  • Demonstration of simple, cheap, precise, and effective muscle wounding protocols in larval zebrafish.
  • Successful monitoring of muscle stem cell and immune cell ingression into damaged areas.
  • Visualization of skeletal muscle fiber regeneration over an extended timecourse in individual subjects.

Conclusions:

  • The described protocols provide a robust and adaptable model for studying muscle regeneration.
  • This approach enhances understanding by enabling detailed analysis of individual regeneration responses.
  • The zebrafish model offers significant potential for advancing research in muscle repair and regenerative therapies.