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Related Concept Videos

Skeletal Muscle Anatomy00:55

Skeletal Muscle Anatomy

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Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
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Related Experiment Video

Updated: Aug 4, 2025

3D Ultrasound Imaging: Fast and Cost-effective Morphometry of Musculoskeletal Tissue
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Three-Dimensional Imaging Analysis for Skeletal Muscle.

Smrithi Karthikeyan1, Kyutae Kim1, Yoko Asakura1

  • 1Stem Cell Institute, University of Minnesota Medical School, Minneapolis, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2023
PubMed
Summary

This study outlines a 3-D imaging protocol for skeletal muscle regeneration. The workflow uses confocal microscopy and ImageJ, Ilastik, and Imaris software for detailed visualization of muscle tissue.

Keywords:
3D imagingAngiogenesisEndothelial cellMuscle regenerationMuscle stem cellMuscular dystrophyMyogenesisSatellite cellSkeletal muscleTissue clearing

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Area of Science:

  • Cell Biology
  • Tissue Engineering
  • Biomedical Imaging

Background:

  • Skeletal muscle possesses significant regenerative capacity, driven by complex cellular interactions during homeostasis and injury.
  • Understanding muscle regeneration requires advanced three-dimensional (3-D) imaging techniques.
  • Existing 3-D imaging protocols have predominantly focused on neural tissues, leaving a gap for muscle-specific methods.

Purpose of the Study:

  • To establish a comprehensive protocol for generating 3-D images of skeletal muscle.
  • To detail a workflow for visualizing muscle regeneration processes in three dimensions.
  • To provide a reproducible method for spatial analysis of skeletal muscle tissue.

Main Methods:

  • Utilizing confocal microscopy to acquire spatial data from skeletal muscle tissue.
  • Employing ImageJ, Ilastik, and Imaris software for image processing and 3-D rendering.
  • Implementing computational image analysis for segmentation and visualization of cellular structures.

Main Results:

  • A standardized workflow for 3-D skeletal muscle imaging was successfully developed.
  • The protocol enables detailed visualization of the complex cellular architecture within muscle tissue.
  • Software tools like ImageJ, Ilastik, and Imaris demonstrated powerful segmentation capabilities for muscle analysis.

Conclusions:

  • The developed protocol provides a valuable tool for studying skeletal muscle regeneration in 3-D.
  • This method enhances the understanding of cellular dynamics crucial for muscle repair and homeostasis.
  • The protocol's reliance on accessible software facilitates its adoption in research settings.