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Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.)
Truyen Quach1, Hanh Nguyen1, Olivia Meyer2
1Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, USA.
Abstract:
Cas9-based genome editing is a powerful genetic tool for loci specifically targeted for genome modification. This chapter describes up-to-date protocols using Cas9-based genome editing technology, including vector construction with GoldenBraid assembly, Agrobacterium-mediated soybean transformation, and identification of editing in the genome.
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