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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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A CRISPR/Cas9 Protocol for Target Gene Editing in Barley.

Qiantao Jiang1,2, Qiang Yang3,4, Wendy Harwood5

  • 1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, Sichuan, China. qiantaojiang@sicau.edu.cn.

Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2023
PubMed
Summary

We present a precise genome editing protocol for barley, a key model for wheat functional genomics. This CRISPR/Cas9 method enables targeted gene modification, advancing our understanding of crop genetics.

Keywords:
CRISPR/Cas9Genome editingHordeum vulgareT-DNA-free

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Area of Science:

  • Agricultural Science
  • Genetics
  • Molecular Biology

Background:

  • Traditional gene function studies rely on natural genetic variation or random mutagenesis, limiting research depth.
  • Precise genome modification is crucial for understanding gene function in crops.
  • Barley serves as a vital model organism for functional genomic analysis in common wheat.

Purpose of the Study:

  • To detail an effective protocol for genome editing in barley using the CRISPR/Cas9 system.
  • To establish a reliable method for precise gene modification in a key cereal model species.

Main Methods:

  • Utilizing the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system for targeted genome modification.
  • Developing and optimizing a protocol for efficient gene editing in barley.

Main Results:

  • The presented protocol allows for precise and predictable modification of the barley genome.
  • The effectiveness of the CRISPR/Cas9-mediated gene editing method in barley has been previously validated.

Conclusions:

  • The developed barley gene editing protocol is essential for advancing wheat functional genomics.
  • This precise genome editing system facilitates in-depth research into gene function in important cereal crops.