CRISMERE Chromosome Engineering in Mouse and Rat.

Area of Science:

• Genomic Engineering
• Molecular Biology
• Gene Editing Technologies

Background:

• CRISPR/Cas9 technology has revolutionized genome manipulation.
• Simultaneous double-strand breaks offer new possibilities for genomic alterations.

Purpose of the Study:

• To describe the CRISMERE protocol for generating chromosomal rearrangements.
• To detail the validation of these rearrangements in mouse and rat models.

Main Methods:

• Utilizing CRISPR/Cas9 to induce two simultaneous double-strand breaks.
• Generating deletions, inversions, and duplications of specific genomic segments.
• Validating the generated chromosomal rearrangements.

Main Results:

• Successful generation of mouse and rat lines with defined genomic rearrangements.
• Demonstration of CRISMERE's capacity for precise genomic segment manipulation.
• Validation of generated genetic configurations.

Conclusions:

• CRISMERE is an effective method for creating specific chromosomal rearrangements using CRISPR/Cas9.
• These rearrangements are valuable for modeling diseases, studying genomic organization, and developing genetic tools.
• The protocol provides a comprehensive guide for generating and validating these novel genetic configurations.