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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Aug 4, 2025

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Gene Targeting in Rat Embryonic Stem Cells.

Hongsheng Men¹, Daniel J Davis², Elizabeth C Bryda^3,2

¹Rat Resource and Research Center, University of Missouri, Columbia, MO, USA. menho@missouri.edu.

Methods in Molecular Biology (Clifton, N.J.)

|March 30, 2023

Summary

This summary is machine-generated.

Gene targeting in rat embryonic stem (ES) cells enables the creation of genetically modified rat models. This chapter details the essential procedures for achieving precise gene modifications in rat ES cells.

Keywords:

ES cell selection Gene targeting Nucleofection Rat ES cells

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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

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Last Updated: Aug 4, 2025

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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Rat germline-competent embryonic stem (ES) cell lines have been available since 2008.
Genome targeting technology in rats has advanced significantly.
Rat models are crucial for biomedical research.

Purpose of the Study:

To provide a comprehensive overview of gene targeting procedures in rat ES cells.
To facilitate the generation of genetically engineered rat models.
To serve as a guide for researchers utilizing rat ES cell technology.

Main Methods:

Detailed explanation of gene targeting techniques in rat ES cells.
Discussion of genome editing tools and strategies.
Protocols for cell culture and genetic manipulation.

Main Results:

Successful generation of rat models with targeted mutations.
Demonstration of the efficacy of ES cell-based genome targeting.
Establishment of reliable methods for genetic modification in rats.

Conclusions:

Rat ES cell-based genome targeting is a powerful technology.
This chapter provides essential procedural knowledge for researchers.
Advancements in rat genetic engineering are critical for disease modeling.