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Updated: Aug 4, 2025

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
Published on: August 23, 2014
Rat Embryonic Stem Cell Transgenesis
Elizabeth C Bryda1, Hongsheng Men2, Barbara J Stone3
1University of Missouri, Rat Resource and Research Center, Columbia, MO, USA. brydae@missouri.edu.
Abstract:
The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.

