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Rat Embryonic Stem Cell Transgenesis.
Elizabeth C Bryda1, Hongsheng Men2, Barbara J Stone3
1University of Missouri, Rat Resource and Research Center, Columbia, MO, USA. brydae@missouri.edu.
Methods in Molecular Biology (Clifton, N.J.)
|March 30, 2023
Summary
Generating genetically modified rat models is now more feasible. Researchers have developed methods for culturing and manipulating rat embryonic stem cells (ESCs) to create chimeric rats capable of transmitting genetic modifications.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Genetics
Background:
- Rat models are crucial for biomedical research.
- Genetic manipulation of rats is essential for disease modeling.
- Reliable germline competent rat embryonic stem cell (ESC) lines are needed.
Purpose of the Study:
- To describe the process of generating chimeric rats using genetically modified ESCs.
- To establish a method for creating new rat models with specific genetic alterations.
Main Methods:
- Culturing of rat ESCs.
- Microinjection of ESCs into rat blastocysts.
- Surgical and non-surgical embryo transfer to surrogate dams.
Main Results:
- Successful production of chimeric rats.
- Demonstrated potential for germline transmission of genetic modifications.
Conclusions:
- The described methods facilitate the generation of genetically engineered rat models.
- This technique provides a valuable tool for advancing rat-based research.
