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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

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Related Experiment Video

Updated: Aug 4, 2025

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Rat Embryonic Stem Cell Transgenesis.

Elizabeth C Bryda¹, Hongsheng Men², Barbara J Stone³

¹University of Missouri, Rat Resource and Research Center, Columbia, MO, USA. brydae@missouri.edu.

Methods in Molecular Biology (Clifton, N.J.)

|March 30, 2023

Summary

This summary is machine-generated.

Generating genetically modified rat models is now more feasible. Researchers have developed methods for culturing and manipulating rat embryonic stem cells (ESCs) to create chimeric rats capable of transmitting genetic modifications.

Keywords:

Blastocyst Chimera Embryo transfer Embryonic stem cells NSET Rat Transgenesis

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Area of Science:

Developmental Biology
Stem Cell Biology
Genetics

Background:

Rat models are crucial for biomedical research.
Genetic manipulation of rats is essential for disease modeling.
Reliable germline competent rat embryonic stem cell (ESC) lines are needed.

Purpose of the Study:

To describe the process of generating chimeric rats using genetically modified ESCs.
To establish a method for creating new rat models with specific genetic alterations.

Main Methods:

Culturing of rat ESCs.
Microinjection of ESCs into rat blastocysts.
Surgical and non-surgical embryo transfer to surrogate dams.

Main Results:

Successful production of chimeric rats.
Demonstrated potential for germline transmission of genetic modifications.

Conclusions:

The described methods facilitate the generation of genetically engineered rat models.
This technique provides a valuable tool for advancing rat-based research.