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RNA helicase DDX5-induced circPHF14 promotes gastric cancer cell progression

Jia Wang1,2, Chunjie Han3, Jinsheng Wang4,5

  • 1Department of Immunology, Changzhi Medical College, Changzhi 046000, Shanxi, China.

Aging
|March 30, 2023
PubMed

Insights

The RNA helicase DDX5 promotes stomach cancer growth and invasion by upregulating circular RNAs (circRNAs), including circPHF14. This highlights DDX5-induced circRNAs as potential therapeutic targets for gastric cancer.

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • DDX5 is a conserved RNA helicase involved in RNA processing and gene expression.
  • Dysregulation of DDX5 is linked to various cancers, including gastric cancer (GC).
  • Circular RNAs (circRNAs) are emerging as key players in pathological processes, including tumorigenesis.

Purpose of the Study:

  • To investigate the role of DDX5 in regulating circRNA expression in gastric cancer.
  • To determine the functional significance of DDX5-induced circRNAs in GC cell growth and invasion.
  • To identify specific circRNAs regulated by DDX5 in GC.

Main Methods:

  • Analysis of DDX5 expression in gastric cancer tissues.
  • Genome-wide circRNA sequencing to identify DDX5-regulated circRNAs.
  • Functional assays to assess the role of circPHF14 in GC cell growth and tumorigenesis.

Main Results:

  • DDX5 was significantly upregulated in gastric cancer tissues and promoted GC cell growth and invasion.
  • DDX5 overexpression induced a broad range of circRNAs in GC cells.
  • circPHF14 was identified as a key DDX5-induced circRNA essential for the growth and tumorigenesis of DDX5-positive GC cells.

Conclusions:

  • DDX5 plays a critical role in gastric cancer progression by modulating the circRNA landscape.
  • DDX5-induced circRNAs, exemplified by circPHF14, are crucial for the growth of DDX5-positive gastric cancer.
  • Targeting DDX5-regulated circRNAs represents a promising therapeutic strategy for gastric cancer.
Keywords:
gastric cancertumor

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