Expression Pattern and Value of Brain-Derived Neurotrophic Factor in Periodontitis
He Chen1, Xubo Feng2, Qian Yang2
1Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China.
International Dental Journal
|March 30, 2023
Summary
Brain-derived neurotrophic factor (BDNF) is hypermethylated and downregulated in periodontitis. This suggests BDNF may serve as a potential biomarker and therapeutic target for periodontitis treatment.
Area of Science:
- Periodontal medicine
- Molecular biology
- Genomics
Background:
- Periodontitis is a prevalent human disease with rising incidence.
- Brain-derived neurotrophic factor (BDNF) is vital for periodontal tissue regeneration.
- Further research is needed on BDNF's role in periodontitis.
Purpose of the Study:
- Investigate the expression and methylation status of BDNF in periodontitis.
- Explore the molecular functions and clinical relevance of BDNF in periodontitis.
Main Methods:
- Utilized Gene Expression Omnibus (GEO) database for RNA expression and methylation data.
- Performed bioinformatics analysis to identify BDNF's downstream functions.
- Confirmed BDNF expression levels using Reverse transcription Quantitative real-time polymerase chain reaction (RT-qPCR).
Main Results:
- BDNF showed hypermethylation and downregulation in periodontitis tissues.
- Protein-protein interaction network identified interacting genes.
- Functional analysis linked BDNF to cytoplasmic dynein complex, glutathione transferase activity, and metabolic pathways.
- BDNF expression correlated with B cell and CD4+ T cell infiltration.
Conclusions:
- BDNF is hypermethylated and downregulated in periodontitis.
- BDNF presents potential as a biomarker and therapeutic target for periodontitis.
