Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolving Roles of Primary Cilia in CNS Development and Neural Circuit Function: From Human Disease to Molecular Underpinnings.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

SIX1 branchio-oto-renal syndrome variants have different effects on embryonic craniofacial gene expression and cartilage formation.

Development (Cambridge, England)·2026
Same author

Magnetoelectric microrobots for spinal cord injury regeneration.

Nature materials·2026
Same author

Altair-dvOPM: an open-access platform for large-field three-dimensional tissue imaging.

bioRxiv : the preprint server for biology·2026
Same author

Adapting in ovo RNAi as a tool to study gene function during neural circuit formation in the cerebellum.

Journal of neuroscience methods·2026
Same author

Gpnmb defines a phagocytic state of microglia linked to cell death in prion disease mouse model.

Nature communications·2026
Same journal

Author Correction: Sustained nitric oxide production by engineered E. coli remodels the tumor microenvironment and potentiates immunotherapy.

Nature biotechnology·2026
Same journal

Quantum computing in transition.

Nature biotechnology·2026
Same journal

Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates.

Nature biotechnology·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026
See all related articles

Related Experiment Video

Updated: Aug 4, 2025

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
08:13

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Published on: April 8, 2019

17.5K

Reflective multi-immersion microscope objectives inspired by the Schmidt telescope.

Fabian F Voigt1,2,3, Anna Maria Reuss4,5, Thomas Naert6

  • 1Brain Research Institute, University of Zurich, Zurich, Switzerland. fvoigt@fas.harvard.edu.

Nature Biotechnology
|March 30, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel multi-immersion Schmidt objective for advanced microscopy. This innovative lens design enables imaging of large, cleared samples across diverse immersion media with high numerical aperture and long working distance.

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.3K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K

Related Experiment Videos

Last Updated: Aug 4, 2025

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
08:13

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Published on: April 8, 2019

17.5K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.3K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K

Area of Science:

  • Microscopy
  • Optical Engineering
  • Biophysics

Background:

  • Advanced imaging techniques require microscope objectives with large fields of view (FOV), long working distances (WD), and high numerical apertures (NA).
  • Conventional lens-based objectives face challenges in compatibility with diverse immersion media, limiting their versatility for imaging large, cleared samples.

Purpose of the Study:

  • To introduce a novel multi-immersion Schmidt objective designed to overcome the limitations of conventional objectives.
  • To demonstrate the objective's compatibility with various immersion media and its performance in high-resolution imaging applications.

Main Methods:

  • The study introduces a multi-immersion Schmidt objective, comprising a spherical mirror and an aspherical correction plate.
  • A multi-photon variant of the Schmidt objective was tested for its imaging capabilities.

Main Results:

  • The multi-immersion Schmidt objective demonstrated compatibility with all tested homogeneous immersion media.
  • The objective achieved a high numerical aperture (NA) of 1.08 at a refractive index of 1.56, with a 1.1-mm FOV and an 11-mm WD.
  • Successful imaging of cleared samples in diverse media (air, water, benzyl alcohol/benzyl benzoate, dibenzyl ether, ethyl cinnamate) and in vivo neuronal activity in zebrafish was achieved.

Conclusions:

  • The multi-immersion Schmidt objective offers a versatile solution for imaging large, cleared samples across a wide range of immersion media.
  • The design principles can be extended to various microscopy modalities, including wide-field, confocal, and light-sheet microscopy.