Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

2.5K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.5K
Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

3.7K
 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
3.7K
Plant Cell Wall01:07

Plant Cell Wall

4.4K
Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
4.4K
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

2.8K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
2.8K
The Phragmoplast01:59

The Phragmoplast

5.2K
Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...
5.2K
Morphogenesis02:19

Morphogenesis

28.5K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
28.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Natural variation in SlGRF10 reveals a role in regulating tomato fruit weight.

Plant physiology·2026
Same author

PERSPECTIVE: organelle positioning as a principle of metabolic regulation and stress tolerance.

The Plant journal : for cell and molecular biology·2026
Same author

Genomic insights into the improvement of Chinese fir from ancient domestication continuum to modern breeding.

Nature communications·2026
Same author

Lack of GDSL motif-containing proteins increases drought tolerance by altering the stomatal cuticle in Arabidopsis.

The Plant cell·2026
Same author

Arabidopsis growth and reproduction require UDP-arabinofuranose import into the Golgi apparatus.

Plant physiology·2026
Same author

Protein-saliva interactions govern the structure and lubrication of salivary films.

Journal of colloid and interface science·2026

Related Experiment Video

Updated: Aug 4, 2025

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

13.4K

DEFECTIVE KERNEL1 regulates cellulose synthesis and affects primary cell wall mechanics.

Lazar Novaković1,2, Gleb E Yakubov3, Yingxuan Ma1,4

  • 1School of BioSciences, University of Melbourne, Parkville, VIC, Australia.

Frontiers in Plant Science
|March 31, 2023
PubMed
Summary

DEFECTIVE KERNEL1 (DEK1), a mechanosensitive protease, regulates plant cell wall mechanics and cellulose synthesis. DEK1 influences cell wall stiffness and cellulose microfibril thickness in Arabidopsis cotyledons.

Keywords:
DEFECTIVE KERNEL1cell wallcellulosecellulose synthase complexmechanical properties

More Related Videos

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
09:27

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

Published on: May 10, 2016

8.2K
Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
10:39

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Published on: May 13, 2014

41.0K

Related Experiment Videos

Last Updated: Aug 4, 2025

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

13.4K
High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
09:27

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications

Published on: May 10, 2016

8.2K
Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
10:39

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Published on: May 13, 2014

41.0K

Area of Science:

  • Plant Biology
  • Cell Biology
  • Biochemistry

Background:

  • The plant cell wall is crucial for cell shape, growth, and environmental interactions.
  • Mechanosensitive proteins play key roles in sensing and responding to mechanical cues.

Purpose of the Study:

  • To investigate the role of the putative mechanosensitive Cys-protease DEFECTIVE KERNEL1 (DEK1) in regulating plant cell wall properties and cellulose synthesis.
  • To elucidate DEK1's function in Arabidopsis thaliana during early development.

Main Methods:

  • Analysis of DEK1's influence on mechanical properties of primary cell walls.
  • Investigation of DEK1's role in regulating cellulose synthesis in epidermal tissues.
  • Examination of DEK1's effects on cellulose synthase complexes (CSCs) and associated regulatory proteins.

Main Results:

  • DEK1 significantly influences the mechanical properties of primary cell walls.
  • DEK1 is identified as a key regulator of cellulose synthesis in Arabidopsis epidermal cells.
  • DEK1 modulates the biosynthetic properties of CSCs, potentially via interactions with regulatory proteins.
  • Altered cell wall stiffness and cellulose microfibril bundle thickness were observed in DEK1-modulated lines.

Conclusions:

  • DEK1 is an important regulator of cellulose synthesis and primary cell wall mechanics in Arabidopsis.
  • DEK1's function in modulating CSCs highlights its significance in plant development and cell wall integrity.