Related Experiment Video

Updated: Aug 4, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.4K

MicroRNA-34c-5p Reduces Malignant Properties of Lung Cancer Cells through Regulation of TBL1XR1/Wnt/β-catenin

Weiqiang Lai1, Yonghong Yue2, Ganhua Zeng1

  • 1Department of Thoracic Surgery, Ganzhou People's Hospital, Ganzhou, Jiangxi, 341000, P.R. China.

Current Molecular Medicine
|March 31, 2023
PubMed
Abstract

Insights

MicroRNA-34c-5p (miR-34c-5p) inhibits lung cancer progression by downregulating transducin β-like 1 X-linked receptor 1 (TBL1XR1). This study shows miR-34c-5p represses malignant behaviors and EMT via TBL1XR1, suggesting its therapeutic potential.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Regulation

Background:

  • Lung cancer is a leading cause of cancer mortality.
  • MicroRNAs (miRNAs) play crucial roles in cancer progression.
  • The specific function and mechanism of miR-34c-5p in lung cancer remain largely undetermined.

Purpose of the Study:

  • To investigate the role of miR-34c-5p in the malignant behaviors of lung cancer cells.
  • To elucidate the underlying molecular mechanism involving transducin β-like 1 X-linked receptor 1 (TBL1XR1).

Main Methods:

  • Differential expression analysis using public databases.
  • qRT-PCR and Western blot to assess miR-34c-5p and TBL1XR1 expression.
  • Cell transfection, viability (CCK-8), migration (scratch), and invasion (Matrigel-Transwell) assays.
  • Dual-luciferase reporter gene assay to confirm the interaction between miR-34c-5p and TBL1XR1.
  • Analysis of Wnt/β-catenin signaling and epithelial-mesenchymal transition (EMT) markers.

Main Results:

  • miR-34c-5p was significantly downregulated, while TBL1XR1 was upregulated in lung cancer cells.
  • miR-34c-5p directly targets and represses TBL1XR1 expression.
  • Overexpression of miR-34c-5p inhibited lung cancer cell proliferation, migration, invasion, Wnt/β-catenin signaling, and EMT.
  • TBL1XR1 upregulation counteracted the inhibitory effects of miR-34c-5p.

Conclusions:

  • miR-34c-5p acts as a tumor suppressor in lung cancer by inhibiting malignant behaviors.
  • The mechanism involves the downregulation of TBL1XR1, Wnt/β-catenin signaling, and EMT.
  • miR-34c-5p represents a potential therapeutic target for lung cancer treatment.
Keywords:
Lung cancerTBL1XR1.invasionmicroRNA-34c-5pmigrationproliferation

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
The Soft Agar Colony Formation Assay08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

112.0K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.4K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K