Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation of IgE from normal mouse serum.

S B Lehrer

    Immunology
    |January 1, 1979
    PubMed
    Summary

    Researchers successfully isolated mouse immunoglobulin E (IgE) from normal mouse serum using an immunoadsorbent technique. This breakthrough provides a crucial source of mouse IgE for allergy research and understanding immune responses.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Fish Allergy: Is It a Real Problem with Surimi-Based Products?

    International archives of allergy and immunology·2021
    Same author

    Shellfish allergy.

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2010
    Same author

    Do shrimp-allergic individuals tolerate shrimp-derived glucosamine?

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2006
    Same author

    Structural, immunological and functional properties of natural recombinant Pen a 1, the major allergen of Brown Shrimp, Penaeus aztecus.

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2006
    Same author

    Risks of allergic reactions to biotech proteins in foods: perception and reality.

    Allergy·2005
    Same author

    Environmental exposure characterization of fish processing workers.

    The Annals of occupational hygiene·2005

    Area of Science:

    • Immunology
    • Allergy Research
    • Biochemistry

    Background:

    • Mouse immunoglobulin E (IgE) is critical for studying allergic reactions.
    • Previous methods for obtaining mouse IgE were limited, hindering research.
    • A reliable source of purified mouse IgE is needed for in-depth analysis.

    Purpose of the Study:

    • To develop a method for isolating and purifying mouse IgE from normal mouse serum.
    • To characterize the isolated mouse IgE.
    • To assess the functional properties of purified mouse IgE in allergic models.

    Main Methods:

    • Production of rabbit antiserum against mouse IgE.
    • Preparation of an anti-mouse IgE immunoadsorbent for IgE isolation from normal mouse serum.
    • Sequential purification steps including ammonium sulfate precipitation, affinity chromatography, gel filtration, ion exchange chromatography, and electrophoresis.
    • Characterization using immunoelectrophoresis and immunoprecipitation.
    • Assessment of passive cutaneous anaphylaxis (PCA) inhibition.

    Main Results:

    • Successfully isolated and purified mouse IgE from normal mouse serum with an estimated concentration of 1.15 microgram/ml.
    • Purified IgE demonstrated a single component upon immunoelectrophoresis and immunoprecipitation.
    • The isolated IgE could inhibit 48-hour passive cutaneous anaphylaxis reactions, suggesting reagin-like binding to mouse skin.

    Conclusions:

    • Mouse IgE can be effectively isolated from normal mouse serum using immunoadsorbent techniques.
    • The availability of purified mouse IgE facilitates further research using mouse models of allergic reactions.
    • This method provides a valuable resource for understanding IgE-mediated immunity and allergy.

    Related Experiment Videos