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Sphingomonas cremea sp. nov., isolated from ginseng soil
Yuna Park1,2, Qingzhen Liu3,4,5, Soohyun Maeng1
1Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 01797, Republic of Korea.
Summary
A new bacterium, Sphingomonas cremea sp. nov. strain G124T, was discovered in Korean ginseng soil. This Gram-negative bacterium expands the known diversity within the Sphingomonas genus.
Area of Science:
- Microbiology
- Bacteriology
- Phylogenetics
Background:
- The genus Sphingomonas comprises diverse bacteria found in various environments.
- Understanding bacterial diversity is crucial for ecological and biotechnological applications.
Purpose of the Study:
- To isolate and characterize a novel bacterial strain from ginseng soil.
- To determine the phylogenetic and chemotaxonomic properties of the isolate.
- To formally propose a new species within the genus Sphingomonas.
Main Methods:
- Isolation of strain G124T from ginseng soil.
- Phylogenetic analysis using 16S rRNA gene sequences.
- Chemotaxonomic analysis including respiratory quinones, polar lipids, and fatty acids.
- Genomic analysis including genome size and G+C content.
- Calculation of average nucleotide identity and digital DNA-DNA hybridization.
Main Results:
- Strain G124T is a Gram-negative, aerobic, rod-shaped, non-motile bacterium.
- Phylogenetic analysis placed strain G124T within the genus Sphingomonas, closely related to S. rhizophila.
- Chemotaxonomic data (major quinone, polar lipids, fatty acids) supported its classification.
- Genomic analysis revealed a genome size of 2,549,305 bp and a G+C content of 62.0 mol%.
- Low ANI and dDDH values confirmed its status as a distinct species.
Conclusions:
- Strain G124T represents a novel species of the genus Sphingomonas.
- The proposed name for this new species is Sphingomonas cremea sp. nov.
- The type strain is G124T (=KACC 21691T=LMG 31729T).
