Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

151
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
151
Key Techniques in Microbiology01:29

Key Techniques in Microbiology

162
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
162
Healthcare Associated Infections II: Preventive Measures01:22

Healthcare Associated Infections II: Preventive Measures

2.7K
Essential infection prevention measures are based on the knowledge of the infection chain, the modes of transmission in healthcare settings, and the use of the best practices in all healthcare settings. Compulsory public reporting of healthcare-associated infection rates is needed to allow individuals and the community to make informed choices regarding selecting a healthcare facility.
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
2.7K
Transmission-based Precautions I: Contact, Enteric, and Droplets01:17

Transmission-based Precautions I: Contact, Enteric, and Droplets

3.8K
Transmission-based precautions are for patients known to be infected or suspected to be infected or colonized with organisms that pose a significant risk to others. Some transmission-based precautions include contact, enteric, and droplet.
Contact Precautions:
Contact precautions are the measures taken to prevent the transmission of infectious agents, especially epidemiologically important microorganisms such as MRSA or influenza, primarily transmitted through direct or indirect contact with an...
3.8K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

51
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
51
Transmission-based Precautions II: Airborne and Protective Environment01:25

Transmission-based Precautions II: Airborne and Protective Environment

1.3K
Transmission-based precautions are for patients infected or suspected to be infected (or colonized) with organisms posing a significant risk to others. The transmission precautions include airborne and protective environment precautions.
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Detection and Surveillance of the SARS-CoV-2 Variant BA.3.2 - Worldwide, November 2024-February 2026.

MMWR. Morbidity and mortality weekly report·2026
Same author

Healthcare wastewater surveillance: methodological considerations for sampling, feasibility, and implementation.

Journal of water and health·2026
Same author

PaedIatric caNcelation ratEs And PerioPerative clinicaL Evaluation (PINEAPPLE): A UK Prospective Multi-Center Observational Cohort Study.

Paediatric anaesthesia·2026
Same author

Estimated COVID-19 Periodicity and Correlation with SARS-CoV-2 Spike Protein S1 Antigenic Diversity, United States.

Emerging infectious diseases·2025
Same author

When the Household is the Utility: Ensuring Equitable Water Service for Rural US Communities Served by Decentralized Water Systems.

ACS ES&T water·2025
Same author

Population Ecology-Quantitative Microbial Risk Assessment (QMRA) Model for Antibiotic-Resistant and Susceptible <i>E. coli</i> in Recreational Water.

Environmental science & technology·2025
Same journal

Correction to "Marine Scrubbers vs Low-Sulfur Fuels: A Comprehensive Well-To-Wake Life Cycle Assessment Supported by Measurements Aboard an Ocean-Going Vessel".

Environmental science & technology·2026
Same journal

Emissions and Cost Trade-Offs of Time-Matched Clean Electricity Procurement under Interannual Weather Variability: A Case Study of Hydrogen Production.

Environmental science & technology·2026
Same journal

Divergent Thermal Feedbacks of Urbanization and Greening Modulate the Urban Heat Island in 21st-Century China.

Environmental science & technology·2026
Same journal

Friction-Mediated Transfer of Low Molecular Weight Chemicals from Consumer Mats to Fabrics: Insights for Dermal Exposure.

Environmental science & technology·2026
Same journal

Molecular Drivers of Contrasting Photoreactivity in Extracellular versus Intracellular Organic Matter from Chlorophyta and Cyanobacteria.

Environmental science & technology·2026
Same journal

Effective Precipitate Cleaning with a Reversible Flow Cell Sustains Stable Energy Intensity for Oceanic CO<sub>2</sub> Removal.

Environmental science & technology·2026
See all related articles

Related Experiment Video

Updated: Aug 4, 2025

Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 3. Aerobiology
11:13

Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 3. Aerobiology

Published on: October 3, 2016

14.4K

Informing Building Strategies to Reduce Infectious Aerosol Transmission Risk by Integrating DNA Aerosol Tracers with

Nicholas Clements1, Ilan Arvelo2, Phil Arnold2

  • 1Paul M. Rady Department of Mechanical Engineering, University of Colorado at Boulder, Boulder, Colorado 80309, United States.

Environmental Science & Technology
|March 31, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a DNA tracer method to estimate airborne infection risk from pathogens like SARS-CoV-2. This approach helps design safer buildings and assess the effectiveness of interventions such as ventilation and air filtration.

Keywords:
aerosol traceraerosol transmissioninfectious aerosolqmrarisk modeling

More Related Videos

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.9K
Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
09:30

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

3.5K

Related Experiment Videos

Last Updated: Aug 4, 2025

Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 3. Aerobiology
11:13

Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 3. Aerobiology

Published on: October 3, 2016

14.4K
Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
06:11

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

1.9K
Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
09:30

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

3.5K

Area of Science:

  • Environmental Health
  • Infectious Disease Epidemiology
  • Aerosol Science

Background:

  • Estimating airborne infectious disease transmission risk is crucial for designing protective indoor environments.
  • Existing models often require complex measurements of pathogen concentrations.
  • Aerosol-based tracers offer a potential surrogate for assessing transmission risk.

Purpose of the Study:

  • To develop and evaluate a method for scaling a quantitative microbial risk assessment (QMRA) model using aerosolized DNA tracers.
  • To assess the impact of various interventions on infection risk using this tracer-scaled QMRA model.
  • To estimate the risk reduction provided by HEPA air cleaners in an ambulatory care setting.

Main Methods:

  • A SARS-CoV-2 bulk aerosol QMRA model was scaled using synthetic DNA tracer concentrations.
  • Point-of-emission ratios linked tracer and respiratory aerosol characteristics.
  • Infection risk was scaled with time-integrated tracer concentrations measured by filter samplers, accounting for pathogen inactivation.
  • Model performance was evaluated via scenario testing and application to real-world measurement data.

Main Results:

  • The tracer-scaled QMRA model successfully estimated infection risk and the impact of interventions like ventilation, occupancy, and masking.
  • Application to ambulatory care room data demonstrated the model's ability to quantify risk reduction from HEPA air cleaner operation.
  • The method provides a simplified approach to risk estimation and evaluation of mitigation strategies.

Conclusions:

  • Scaling QMRA models with DNA tracer measurements is a feasible and relatively simple method for estimating airborne infection risk in indoor environments.
  • This approach facilitates the assessment of risk mitigation strategies, including ventilation and air filtration.
  • The developed method supports informed building design and public health interventions against aerosol-transmissible pathogens.