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Updated: Aug 7, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Abstract:
Analysis of aminoacylase-1 (ACY-1) zymograms from 21 species indicated one structural locus coding for one dimeric enzyme. Among the various strains of five laboratory mammals (Mesocricetus auratus, Cricetulus griseus, Mus musculus, Rattus norvegicus, Cavia porcellus) only in Mus musculus-strains genetic variability was found. Crosses segregating for ACY-1b/ACY-1c reveal a three-banded banding pattern typical for the heterozygous state of a dimeric enzyme. Electrophoretic comparisons of all species studied indicate a high degree of phylogenetic variability at this locus.
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