Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

3.4K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.4K
Chemotaxis in E. coli01:27

Chemotaxis in E. coli

43
Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
43

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Macrophages self-generate and refine chemotactic gradients during migration towards complement C5a.

PLoS biology·2026
Same author

Live-cell 3D-SIM of Rift Valley fever virus NSs filaments reveals a polygon web architecture.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

CYRI-B loss promotes enlarged mature focal adhesions and restricts microtubule and ERC1 access to the cell leading edge.

Journal of cell science·2025
Same author

Collective migration: Galvanotaxis in field-tested teams.

Current biology : CB·2025
Same author

Scar/WAVE drives actin protrusions independently of its VCA domain using proline-rich domains.

Current biology : CB·2024
Same author

Biogenesis of lysosome-related organelles complex-2 is an evolutionarily ancient proto-coatomer complex.

Current biology : CB·2024

Related Experiment Video

Updated: Aug 4, 2025

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

7.0K

Competition between chemoattractants causes unexpected complexity and can explain negative chemotaxis.

Adam Dowdell1, Peggy I Paschke2, Peter A Thomason2

  • 1School of Cancer Sciences, University of Glasgow, Garscube Estate, Glasgow G61 1QH, UK; CRUK Beatson Institute, Switchback Road, Glasgow G63 9AE, UK.

Current Biology : CB
|March 31, 2023
PubMed
Summary

Cell migration away from repellents, known as negative chemotaxis, is driven by competition between signaling molecules binding to the same receptor. This competition explains how cells sense and move away from certain chemicals.

Keywords:
chemoattractant breakdownchemorepellentimmune responsesmodelingnegative chemotaxisphosphodiesterasereverse chemotaxis

More Related Videos

C. elegans Chemotaxis Assay
06:28

C. elegans Chemotaxis Assay

Published on: April 27, 2013

46.0K
An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates
06:18

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

Published on: March 25, 2022

4.7K

Related Experiment Videos

Last Updated: Aug 4, 2025

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
10:40

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Published on: November 9, 2017

7.0K
C. elegans Chemotaxis Assay
06:28

C. elegans Chemotaxis Assay

Published on: April 27, 2013

46.0K
An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates
06:18

An Improved Chemotaxis Assay for the Rapid Identification of Rhizobacterial Chemoattractants in Root Exudates

Published on: March 25, 2022

4.7K

Area of Science:

  • Cell Biology
  • Biophysics
  • Developmental Biology

Background:

  • Negative chemotaxis is crucial for processes like nervous system development and inflammation resolution.
  • The molecular mechanisms underlying cell repulsion remain largely unknown.

Purpose of the Study:

  • To elucidate the mechanisms of negative chemotaxis and cell repulsion.
  • To investigate how competing ligands influence cell migration patterns.

Main Methods:

  • Utilized predictive computational modeling to simulate cell behavior.
  • Conducted experimental validation using Dictyostelium discoideum cell assays.

Main Results:

  • Demonstrated that ligand competition for a single receptor induces chemorepulsion.
  • Showed that 8-CPT-cAMP repels cells only in the presence of cAMP.
  • Observed complex migration patterns, including bidirectional movement and arbitrary convergence, when ligands are degraded.
  • Found that combinations of attractants can induce repulsion.

Conclusions:

  • Ligand competition is a key mechanism driving negative chemotaxis and chemorepulsion.
  • Computational models can predict novel biological phenomena in cell migration.
  • Findings have broad implications for understanding cell-cell communication and tissue patterning.