Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

New mitomycin analogs produced by directed biosynthesis.

C A Claridge, J A Bush, T W Doyle

The Journal of Antibiotics

|March 1, 1986

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026

Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025

Same author

Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

Physical review letters·2022

Same author

Constraints on Cosmic Strings Using Data from the Third Advanced LIGO-Virgo Observing Run.

Physical review letters·2021

Same author

Prospects for observing and localizing gravitational-wave transients with Advanced LIGO, Advanced Virgo and KAGRA.

Living reviews in relativity·2020

Same author

GW190521: A Binary Black Hole Merger with a Total Mass of 150 M_{⊙}.

Physical review letters·2020

Same journal

Tsukubacyclinone, a novel angucyclinone compound with anti-inflammatory activity in synovial cell line SW982, produced by Streptomyces sp. K21-0141.

The Journal of antibiotics·2026

Same journal

Macrolide antibiotic action, resistance, and tolerance, and approaches to improve their activity against Gram-negative bacteria.

The Journal of antibiotics·2026

Same journal

Activation of hamiformamide production in the thermotolerant fungus Hamigera avellanea triggered by co-culture with animal immune cells.

The Journal of antibiotics·2026

Same journal

Biosynthesis and enzymology of azaindane natural products.

The Journal of antibiotics·2026

Same journal

Inhibition of Proteus mirabilis growth through gallium-based disruption of iron and heme metabolism.

The Journal of antibiotics·2026

Same journal

Artificial intelligence-based phenotypic screening using Aspergillus oryzae identified a novel antifungal RK-1205-I.

The Journal of antibiotics·2026

See all related articles

Supplementing Streptomyces caespitosus fermentation with primary amines yields novel mitomycin analogs. These Type I and Type II analogs show enhanced activity in prophage induction and against leukemia.

Area of Science:

Microbiology
Medicinal Chemistry
Molecular Biology

Background:

Mitomycin C is a crucial chemotherapeutic agent produced by Streptomyces caespitosus.
Understanding the structural modifications of mitomycin C can lead to novel analogs with improved therapeutic properties.

Purpose of the Study:

To investigate the production of new mitomycin analogs by supplementing the fermentation medium with primary amines.
To characterize the structure and biological activity of these novel analogs.

Main Methods:

Fermentation of Streptomyces caespitosus with various primary amines.
Isolation and structural characterization of produced mitomycin analogs using spectroscopic techniques.
Evaluation of analog activity in prophage induction assays and against L1210 lymphatic leukemia in mice.

Related Experiment Videos

Main Results:

Two new types of mitomycin analogs, Type I and Type II, were successfully produced.
Type I analogs feature C7 amine substitution on the mitosane ring, similar to mitomycin C.
Type II analogs also have C7 substitutions but with a mitomycin B-related ring conformation, including C9a-OH and a methyl-substituted aziridine.
Isolated analogs from methylamine, ethylamine, propylamine, propargylamine, and 2-methylallylamine supplementation demonstrated increased activity in prophage induction and against L1210 leukemia.
No incorporation of secondary amines was observed.

Conclusions:

Supplementation of Streptomyces caespitosus fermentation with primary amines is an effective strategy for generating novel mitomycin analogs.
Type I mitomycin analogs exhibit superior prophage induction and anti-leukemic activity compared to Type II analogs.
Further exploration of amine supplementation may yield additional uncharacterized mitomycin derivatives with therapeutic potential.