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Indirect spectrophotometric method for determining epicillin.

L J Núñez-Vergara, A Roa, J A Squella

    Journal - Association of Official Analytical Chemists
    |March 1, 1986
    PubMed
    Summary
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    A novel UV spectrophotometric assay accurately determines epicillin in capsules and plasma. This method utilizes a specific degradation product, offering a reliable alternative for pharmaceutical and clinical analysis.

    Area of Science:

    • Analytical Chemistry
    • Pharmaceutical Analysis

    Background:

    • Accurate quantification of antibiotics like epicillin is crucial for drug quality control and therapeutic monitoring.
    • Existing analytical methods may have limitations in sensitivity, specificity, or applicability to biological matrices.

    Purpose of the Study:

    • To develop and validate a new UV spectrophotometric assay for epicillin determination.
    • To assess the assay's applicability in both pharmaceutical formulations (capsules) and biological samples (plasma).

    Main Methods:

    • Epicillin was subjected to acidic hydrolysis to generate a quantifiable degradation product.
    • The UV absorptivity of this product was utilized for spectrophotometric quantification.
    • The method was validated through isolation, identification, and recovery studies.

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  • Assays were performed on individual and composite capsule samples.
  • The proposed method was compared against a dc polarographic assay for plasma samples.

    • Main Results:

    • The UV spectrophotometric assay demonstrated reliable determination of epicillin.
    • Successful application of the method for analyzing epicillin in capsule formulations.
    • The assay proved effective for quantifying epicillin in plasma samples.
    • Recovery studies indicated good accuracy and precision.

    Conclusions:

    • The developed UV spectrophotometric assay is a viable and effective method for epicillin quantification.
    • This assay offers a practical alternative for routine analysis of epicillin in pharmaceutical and clinical settings.