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Related Experiment Videos

Determination of nitrate in forages by using selective ion electrode: collaborative study.

M P Carlson, N R Schneider

    Journal - Association of Official Analytical Chemists
    |March 1, 1986
    PubMed
    Summary

    This study validates a potentiometric method for analyzing nitrate in forage samples. The method demonstrated good repeatability and reproducibility, making it suitable for official use in agricultural analysis.

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    Area of Science:

    • Agricultural Chemistry
    • Analytical Chemistry

    Background:

    • Accurate nitrate determination in forages is crucial for animal nutrition and environmental management.
    • Existing methods may have limitations in terms of accuracy, precision, or ease of use.

    Purpose of the Study:

    • To evaluate the performance of a potentiometric method for quantifying nitrate in diverse forage matrices.
    • To assess the method's repeatability and reproducibility across multiple laboratories.

    Main Methods:

    • A potentiometric method utilizing a nitrate-selective electrode was employed.
    • Ten laboratories analyzed blind duplicate forage samples, controls, and standards.
    • Nitrate was extracted using an aqueous aluminum sulfate solution and quantified.

    Main Results:

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    • Nitrate concentrations in forage samples varied from <0.50% to 4.35% KNO3.
    • Repeatability coefficients of variation (CVo) ranged from 1.74% to 3.61%.
    • Reproducibility coefficients of variation (CVx) ranged from 6.92% to 7.66% with 94.5% mean recovery of spiked nitrate.

    Conclusions:

    • The validated potentiometric method provides accurate and precise nitrate quantification in forage.
    • The method's robustness across multiple laboratories supports its adoption for official analysis.
    • This method enhances the reliability of nitrate testing in agricultural settings.