Resolution of mouse hepatic cytochrome P-450 isozymes by chromatofocusing

Constituent cytochrome P-450 isozymes from mouse hepatic microsomes were fractionated by chromatofocusing on Polybuffer Exchanger 94 over the pH range 5.3-8.3 and characterized by polyacrylamide gel electrophoresis. Eight isozymes were detected in fractions from unpretreated mice and seven from phenobarbitone-pretreated animals. Isozyme-containing fractions were reconstituted and shown to produce specific monohydroxy metabolites from both testosterone and delta 9-tetrahydrocannabinol.