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Tissue Homogenization and Cell Lysis

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

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Heterogeneous tissue construction by on-demand bubble-assisted acoustic patterning.

Qinghao Hu^1,2, Xuejia Hu³, Yang Shi⁴

¹Department of Clinical Laboratory, Institute of Medicine and Physics, Renmin Hospital, Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics & Technology, Wuhan University, Wuhan 430072, People's Republic of China. yangyiys@whu.edu.cn.

|April 3, 2023

Summary

This summary is machine-generated.

This study introduces a novel bubble-assisted acoustic method for precise cell patterning, enabling the on-demand construction of complex heterogeneous tissue structures with high accuracy.

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Area of Science:

Biotechnology
Tissue Engineering
Acoustic Manipulation

Background:

Achieving precise control over heterogeneous structure assembly is vital for replicating tissue functions.
Current methods face challenges in high-precision control of cellular arrangement.

Purpose of the Study:

To present an on-demand bubble-assisted acoustic method for active cell patterning.
To demonstrate high-precision heterogeneous structure fabrication for tissue engineering.

Main Methods:

Utilizing acoustic radiation forces and microstreaming from oscillating bubble arrays for active cell patterning.
Employing on-demand bubble arrays for flexible and precise construction of cell patterns (up to 45 μm precision).

Main Results:

Successfully constructed an in vitro model of hepatic lobules using patterned endothelial and hepatic parenchymal cells.
Demonstrated functional viability of the constructed tissue model through urea and albumin secretion, enzymatic activity, and cell proliferation over 5 days.

Conclusions:

The bubble-assisted acoustic approach offers a simple, efficient strategy for on-demand, large-area tissue construction.
This technique shows significant potential for fabricating various tissue models with high precision.