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Updated: Jul 21, 2026

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
Published on: October 10, 2014
Scale-up single chamber of microbial fuel cell using agitator and sponge biocarriers
Sima Malekmohammadi1, Seyed Ahmad Mirbagheri1
1Department of Environmental Engineering, Faculty of Civil Engineering, K. N. Toosi University of Technology, Tehran, Iran.
Abstract:
Despite the high efficiency of microbial fuel cells (MFCs), MFCs cannot be a suitable alternative for treatment plants because of insufficient power generation and tiny reactors. Additionally, the increased reactor size and MFC stack result in a reduction in production power and reverse voltage. In this study, a larger MFC with a volume of 1.5 L has been designed called LMFC. A conventional MFC, called SMFC, with a volume of 0.157 L, was constructed and compared with LMFC. Moreover, the designed LMFC can be integrated with other treatment systems and generate significant electricity. In order to evaluate MFC's ability to integrate with other treatment systems, the LMFC reactor was converted into MFC-MBBR by adding sponge biocarriers. A 9.5 percent increase in reactor volume resulted in a 60 percent increase in power density from 290 (SMFC) to 530 (LMFC). An agitator effect was also investigated for better mixing and circulating substrate, which positively affected the power density by about 18%. Compared with LMFCs, the reactor with biocarriers generated a 28% higher power density. The COD removal efficiency of SMFC, LMFC, and MFC-MBBR reactors after 24 h was 85, 66, and 83%, respectively. After 80 h of operation, the Coulombic efficiency of the SMFC, LMFC, and MFC-MBBR reactors was 20.9, 45.43, and 47.28%, respectively. The doubling of coulombic efficiency from SMFC to LMFC reactor shows the design's success. The reduction of COD removal efficiency in LMFC is the reason for integrating this reactor with other systems, which was compensated by adding biocarriers.
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