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A Vortex Method to Disaggregate Platelets for Correct Counting in Pseudothrombocytopenia.

Jianfeng Zhu1, Lei Luo1, Wei Guo1

  • 1Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion
|April 3, 2023
PubMed
Summary
This summary is machine-generated.

Pseudothrombocytopenia (PTCP) causes falsely low platelet counts. An alternative vortex method effectively disaggregates platelet clumps, providing a reliable platelet count without requiring a second blood draw.

Keywords:
Platelet clumpsPlatelet countsPseudothrombocytopeniaVortex method

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Area of Science:

  • Hematology
  • Clinical Pathology
  • Laboratory Medicine

Background:

  • Pseudothrombocytopenia (PTCP) is an artifactual reduction in platelet count (PLT) due to platelet aggregation in vitro.
  • This phenomenon can lead to misdiagnosis and unnecessary investigations.
  • Current methods to correct PTCP may require additional patient procedures.

Purpose of the Study:

  • To evaluate an alternative vortex method for disaggregating platelet clumps in PTCP specimens.
  • To determine the reliability of the vortex method in obtaining an accurate PLT.
  • To assess the impact of the vortex method on other complete blood count parameters.

Main Methods:

  • A vortex method was applied to 221 PTCP specimens to disaggregate platelet clumps.
  • Platelet counts (PLT) and other complete blood count parameters (MPV, RBCs, Hb, Hct, WBCs) were measured before and after vortexing.
  • Results were compared with 85 specimens treated by the citrate method and 20 control samples.

Main Results:

  • The vortex method significantly increased PLT in specimens with platelet clumps (from 54.3 ± 35.2 × 10^9/L to 157.5 ± 58.8 × 10^9/L, p < 0.05).
  • Other complete blood count parameters (MPV, RBCs, Hb, Hct, WBCs) remained comparable before and after vortexing in PTCP specimens (p > 0.05).
  • No significant changes in complete blood counts were observed in control samples after vortexing.

Conclusions:

  • The vortex method is effective in disaggregating platelet clumps in most PTCP specimens.
  • This technique provides a reliable PLT without the need for a second venous puncture.
  • The vortex method does not adversely affect other essential blood count parameters.