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Updated: Aug 4, 2025

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

In vitro Synthesis of Native, Fibrous Long Spacing and Segmental Long Spacing Collagen

Published on: September 20, 2012

Structural and functional properties of collagen isolated from lumpfish and starfish using isoelectric precipitation

Naveen Kumar Vate^1,2, Przemyslaw Strachowski¹, Ingrid Undeland¹

¹Department of Life Sciences - Food and Nutrition Science, Chalmers University of Technology, 412 96 Gothenburg, Sweden.

Food Chemistry: X

|April 3, 2023

Abstract:

The possibility of replacing the very time and resource demanding salting out (SO) method with isoelectric precipitation (IP) during collagen extraction from common starfish and lumpfish was investigated. The effect of IP on yield, structural and functional properties of the collagens was therefore compared with SO. Application of IP resulted in a higher or similar collagen mass yield compared with SO from starfish and lumpfish, respectively. However, the purity of collagens recovered with IP was lower than those recovered with SO. Replacing SO with IP did not affect polypeptide pattern and tropohelical structural integrity of collagen from the two resources as revealed with SDS-PAGE and FTIR analysis. Thermal stability and fibril formation capacity of collagens recovered with IP were also well preserved. Overall, the results showed that the IP can be a promising resource smart alternative for the classic SO precipitation during collagen extraction from marine resources.

Keywords:

Fibril formation Isoelectric precipitation Lumpfish Marine collagen Starfish

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