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Updated: Aug 4, 2025
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
Salmonella enhances osteogenic differentiation in adipose-derived mesenchymal stem cells
Nuradilla Mohamad-Fauzi1, Claire Shaw1, Soraya H Foutouhi2
1Department of Animal Science, College of Agricultural and Environmental Sciences, University of California, Davis, Davis, CA, United States.
Abstract:
The potential of mesenchymal stem cells (MSCs) for tissue repair and regeneration has garnered great attention. While MSCs are likely to interact with microbes at sites of tissue damage and inflammation, like in the gastrointestinal system, the consequences of pathogenic association on MSC activities have yet to be elucidated. This study investigated the effects of pathogenic interaction on MSC trilineage differentiation paths and mechanisms using model intracellular pathogen Salmonella enterica ssp enterica serotype Typhimurium. The examination of key markers of differentiation, apoptosis, and immunomodulation demonstrated that Salmonella altered osteogenic and chondrogenic differentiation pathways in human and goat adipose-derived MSCs. Anti-apoptotic and pro-proliferative responses were also significantly upregulated (p < 0.05) in MSCs during Salmonella challenge. These results together indicate that Salmonella, and potentially other pathogenic bacteria, can induce pathways that influence both apoptotic response and functional differentiation trajectories in MSCs, highlighting that microbes have a potentially significant role as influencers of MSC physiology and immune activity.
Insights
Pathogenic bacteria like Salmonella can alter how mesenchymal stem cells (MSCs) differentiate and behave. This interaction impacts MSCs' roles in tissue repair and immune responses, revealing microbes as key influencers of MSC physiology.
Area of Science:
- Stem cell biology
- Microbiology
- Immunology
Background:
- Mesenchymal stem cells (MSCs) show great potential for tissue repair and regeneration.
- MSCs likely encounter microbes at sites of inflammation and tissue damage.
- The impact of pathogenic microbes on MSC functions remains largely unknown.
Purpose of the Study:
- To investigate how pathogenic bacteria affect MSC differentiation pathways.
- To elucidate the mechanisms underlying microbial influence on MSCs.
- To examine the effects of *Salmonella enterica* serotype Typhimurium on MSCs.
Main Methods:
- Used human and goat adipose-derived MSCs.
- Challenged MSCs with *Salmonella enterica* serotype Typhimurium.
- Analyzed key markers for differentiation, apoptosis, and immunomodulation.
Main Results:
- *Salmonella* altered osteogenic and chondrogenic differentiation pathways in MSCs.
- Anti-apoptotic and pro-proliferative responses were significantly upregulated in MSCs.
- Microbial challenge influences MSCs' apoptotic responses and differentiation trajectories.
Conclusions:
- Pathogenic bacteria, such as *Salmonella*, can significantly influence MSC physiology.
- Microbes play a potentially significant role in modulating MSC activity and immune function.
- Understanding these interactions is crucial for harnessing MSCs' therapeutic potential.
