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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

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Related Experiment Video

Updated: Aug 4, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

Borderline SARS-CoV-2 patients: the trace behind.

Bojana Mohar Vitezić^1,2, Elena Štefančić², Davorka Repac Antić^1,2

¹Department of Microbiology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.

Expert Review of Molecular Diagnostics

|April 3, 2023

Summary

Retesting borderline SARS-CoV-2 patients with inconclusive PCR results is vital. Nearly 30% of these patients converted to positive within seven days, reducing hospital transmission risks.

Keywords:

COVID-19 testing SARS-CoV-2 RT-PCR borderline SARS CoV-2 patient inconclusive PCR result resampling and retesting

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Last Updated: Aug 4, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

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Published on: June 5, 2021

Area of Science:

Clinical Microbiology
Infectious Disease Diagnostics

Background:

Accurate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection is essential for hospital operations.
Inconclusive PCR results for SARS-CoV-2 can impede infection control and clinical decision-making.

Purpose of the Study:

To evaluate the positivity conversion rate in borderline SARS-CoV-2 patients with inconclusive PCR results.
To assess the clinical utility of follow-up testing within seven days.

Main Methods:

Retrospective analysis of borderline SARS-CoV-2 patients undergoing repeat testing.
Analysis of RT-PCR results from the Clinical Department of Clinical Microbiology.

Main Results:

Out of 247 borderline patients retested, 60 (29.4%) showed conversion from inconclusive to positive RT-PCR results.
A significant proportion of patients initially testing inconclusive were found to be positive upon retesting.

Conclusions:

Retesting patients with inconclusive SARS-CoV-2 PCR results is necessary.
Follow-up testing within seven days identifies additional positive cases, mitigating intrahospital transmission risks.