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Using FastID to analyze complex SNP mixtures from indoor dust.

Kelly A Meiklejohn1, Melissa K R Scheible1, Laura M Boggs1

  • 1Department of Population Health and Pathobiology, North Carolina State University College of Veterinary Medicine, Raleigh, North Carolina, USA.

Journal of Forensic Sciences
|April 3, 2023
PubMed
Summary

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Forensic DNA analysis of indoor dust using massively parallel sequencing (MPS) can identify household occupants. This method successfully detected known individuals in dust samples, offering valuable investigative leads.

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Single nucleotide polymorphisms (SNPs) offer supplementary forensic data to short tandem repeats (STRs).
  • Massively parallel sequencing (MPS) streamlines SNP genotyping.
  • Indoor dust is a promising, often overlooked, source of human DNA for forensic analysis.

Purpose of the Study:

  • To evaluate the efficacy of MPS-genotyped SNPs from indoor dust for detecting known household occupants.
  • To assess the potential of dust DNA analysis for generating investigative leads.

Main Methods:

  • 13 households provided occupant buccal samples and indoor dust samples.
  • Thermo Fisher Scientific Precision ID Identity and Ancestry Panels were used for SNP genotyping.
  • Illumina® chemistry and FastID software were employed for sequencing and mixture analysis.
Keywords:
FastIDMPSSNPsindoor dustinterpretation of DNA mixturesinvestigative leadsmassively parallel sequencingsingle nucleotide polymorphisms

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Main Results:

  • An average of 72% of autosomal SNPs were recovered from dust samples.
  • 93% of known occupants were detected in dust samples and could not be excluded as contributors.
  • Non-contributor alleles were identified in 54% of dust samples.

Conclusions:

  • Human DNA analysis in indoor dust using MPS is a viable method for detecting known household occupants.
  • This technique provides a valuable tool for forensic investigations by identifying potential contributors to DNA mixtures found in dust.