Genome Sequences of Bordetella pertussis Isolates from Outbreaks in Northeastern Mexico

Area of Science:

• Microbiology
• Genomics
• Epidemiology

Background:

• Bordetella pertussis causes whooping cough, a contagious respiratory illness.
• Pertussis outbreaks have been observed globally, necessitating genomic surveillance.
• Understanding bacterial genetic diversity is crucial for effective public health interventions.

Purpose of the Study:

• To determine the draft genome sequences of Bordetella pertussis isolates from recent outbreaks in northeastern Mexico.
• To characterize the genetic lineages and clonal groups of these pertussis isolates.

Main Methods:

• Whole-genome sequencing of four Bordetella pertussis clinical isolates.
• Bioinformatic analysis to identify genetic markers and lineages.
• Phylogenetic analysis to determine clonal relationships.

Main Results:

• The draft genome sequences of four Bordetella pertussis isolates were successfully obtained.
• All isolates belonged to the ptxP3 lineage, a significant genetic group of B. pertussis.
• The isolates were further classified into two major clusters based on variations in the fimH allele.

Conclusions:

• The genomic data provide insights into the evolution and population structure of Bordetella pertussis in Mexico.
• The identified genetic clusters highlight the importance of specific alleles, like fimH, in defining pertussis clones.
• This study contributes to the ongoing surveillance of pertussis and informs strategies for disease control.