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Updated: Aug 7, 2026

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Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
A dot screening procedure on nitrocellulose for detecting anti-idiotopes in hybridoma supernatants
Journal of Immunological Methods
|May 22, 1986
Abstract:
A dot assay on nitrocellulose was developed for screening hybridoma fusions for the presence of anti-idiotopes. The technique involves spotting of hybridoma supernatants onto nitrocellulose filters, and detection of anti-idiotope with an alkaline phosphatase-coupled idiotype.
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