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A dot screening procedure on nitrocellulose for detecting anti-idiotopes in hybridoma supernatants

P Borden, E A Kabat

    Journal of Immunological Methods
    |May 22, 1986
    PubMed

    Abstract:

    A dot assay on nitrocellulose was developed for screening hybridoma fusions for the presence of anti-idiotopes. The technique involves spotting of hybridoma supernatants onto nitrocellulose filters, and detection of anti-idiotope with an alkaline phosphatase-coupled idiotype.

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    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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