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Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for
Junling Wang1, Anna S Kashina2
1University of Pennsylvania, Philadelphia, PA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 3, 2023
Abstract:
Here, we describe the procedure for the expression and purification of recombinant ATE1 from E. coli. This method is easy and convenient and can result in one-step isolation of milligram amounts of soluble enzymatically active ATE1 at nearly 99% purity. We also describe a procedure for the expression and purification of E. coli Arg-tRNA synthetase essential for the arginylation assays described in the next two chapters.

