Related Experiment Videos
A procedure to increase the sensitivity of solid-phase immunoassays
A strategy to increase the sensitivity of antibody detection is described. In this method, streptavidin is used as a cross-bridge between a biotin-conjugated second antibody and a DNA plasmid simultaneously labelled with biotin and 32P. With this simple approach antibodies can be detected with a significantly higher sensitivity as compared to the standard procedures using an 125I-labelled second antibody.
A strategy to increase the sensitivity of antibody detection is described. In this method, streptavidin is used as a cross-bridge between a biotin-conjugated second antibody and a DNA plasmid simultaneously labelled with biotin and 32P. With this simple approach antibodies can be detected with a significantly higher sensitivity as compared to the standard procedures using an 125I-labelled second antibody.