Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...

Fluorescence and Phosphorescence: Instrumentation

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Large Stokes shift fluorophores from <i>meta</i>-substituted zwitterions.

Chemical science·2026

Same author

Stabilizing the Hexacyanotrimethylenecyclopropane Electron Acceptor-Structural and Photophysical Characterization.

Angewandte Chemie (International ed. in English)·2026

Same author

Quinones operate as proton-collecting antennas in energy-transducing membranes.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Validated workflows for preparing and characterizing core-stained and surface-labeled fluorescent polymer particles with simple commercial automation tools.

Analytical and bioanalytical chemistry·2026

Same author

Cross-validation of analytical methods for citrate ligand quantification on upconversion and iron oxide nanoparticles.

Analytical and bioanalytical chemistry·2026

Same author

Aroyl-<i>S</i>,<i>N</i>-ketene acetal-triarylamine bichromophores - intramolecular energy transfer and dual emission upon induced aggregation and encapsulation.

RSC advances·2026

Search research articles

Related Experiment Video

Updated: Aug 4, 2025

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

Frequency-Domain Method for Characterization of Upconversion Luminescence Kinetics.

Lucía Labrador-Páez¹, Jouko Kankare², Iko Hyppänen²

¹Department of Applied Physics, KTH Royal Institute of Technology, SE-10691 Stockholm, Sweden.

The Journal of Physical Chemistry Letters

|April 3, 2023

Summary

The frequency-domain method accurately characterizes lanthanide-doped upconversion nanoparticles (UCNPs) luminescence kinetics. This approach offers a reliable alternative to time-domain methods for complex upconversion processes.

More Related Videos

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

Related Experiment Videos

Last Updated: Aug 4, 2025

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells

Published on: September 16, 2014

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

Area of Science:

Materials Science
Spectroscopy
Nanotechnology

Background:

The frequency-domain (FD) method is an alternative to time-domain (TD) methods for luminophore characterization.
FD methods offer advantages in resolving multiple luminescence lifetime components.
FD methods have not been applied to nonlinear luminescent materials like lanthanide-doped upconversion nanoparticles (UCNPs).

Purpose of the Study:

To investigate the applicability of the FD method for analyzing the luminescence kinetics of UCNPs.
To explore the potential of FD methods for studying complex upconversion processes.
To compare FD method results with TD methods for UCNP characterization.

Main Methods:

A simplified rate-equation model for two-photon energy-transfer upconversion was employed.
Analysis of UCNP luminescence response within the FD method framework.
Experimental validation of the FD method using UCNPs.

Main Results:

The FD method can determine effective decay rates for three critical energy states in UCNPs.
The analysis provides insights into the complex kinetics of upconversion processes.
Experimental results from the FD method showed good agreement with TD methods.

Conclusions:

The FD method is a viable and potentially powerful tool for characterizing UCNP luminescence kinetics.
This study extends the application of FD methods to nonlinear luminescent nanomaterials.
FD methods offer a reliable alternative for analyzing complex upconversion dynamics.