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A simple and highly efficient strategy to induce both paternal and maternal haploids through temperature manipulation
Ze Wang1,2,3,4, Min Chen1, Huan Yang2,3,4
1College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Nature Plants
|April 3, 2023
Summary
Temperature manipulation significantly improves haploid production in crops using centromere-specific histone H3 (CENH3) based inducers. This method enhances both maternal and paternal haploid induction rates, revolutionizing plant breeding strategies.
Area of Science:
- Plant breeding
- Genetics
- Molecular biology
Background:
- Haploid production via outcrossing with inducers is crucial for crop breeding.
- Centromere-specific histone H3 (CENH3) manipulation is a promising strategy for developing haploid inducers.
- Existing CENH3-based inducers like GFP-tailswap face challenges, particularly in efficient maternal haploid induction due to male sterility.
Purpose of the Study:
- To develop a simple and highly effective method for improving both paternal and maternal haploid production using CENH3-based inducers.
- To investigate the independent effects of temperature on pollen vigor and haploid induction efficiency.
- To optimize conditions for enhanced haploid induction rates in crops.
Main Methods:
- Investigating the impact of lower temperatures on pollen vigor and higher temperatures on haploid induction efficiency.
- Utilizing pollen from inducers grown at lower temperatures for pollination, followed by a shift to higher temperatures for induction.
- Adjusting inducer growth temperatures pre- and post-pollination to enhance paternal haploid production.
Main Results:
- Lower temperatures significantly enhance pollen vigor but decrease haploid induction efficiency.
- Higher temperatures reduce pollen vigor but increase haploid induction efficiency.
- Maternal haploid induction rates were improved to approximately 24.8% using the optimized temperature strategy.
- Paternal haploid induction was simplified and enhanced under high-temperature conditions.
Conclusions:
- Temperature is a critical and independent factor influencing both pollen vigor and haploid induction efficiency.
- The described temperature-based method offers a simple yet highly effective approach to improve both maternal and paternal haploid production.
- Findings provide valuable insights for the development and application of CENH3-based haploid inducers in various crops.
