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Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
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FKBP11 improves the malignant property of osteosarcoma cells and acts as a prognostic factor of osteosarcoma

Duo Zeng1,2, Jiayu Li1,2, Xuhui Yuan1,2

  • 1Jiangxi Key Laboratory of Cancer Metastasis and Precision Treatment, Central Laboratory, The First Hospital of Nanchang, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China.

Aging
|April 4, 2023
PubMed
Abstract

Insights

FKBP11 is highly expressed in osteosarcoma and promotes cancer progression. Silencing FKBP11 inhibits osteosarcoma cell invasion, migration, and proliferation, offering a potential new therapeutic target for this bone malignancy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a common adolescent bone cancer with stagnant survival rates despite treatment advances.
  • Messenger RNA (mRNA) presents unique opportunities for targeted drug therapies.
  • Identifying novel prognostic factors and therapeutic targets is crucial for improving osteosarcoma patient outcomes.

Purpose of the Study:

  • To identify a new prognostic factor for osteosarcoma.
  • To provide a novel therapeutic target for osteosarcoma treatment.
  • To investigate the role of FKBP11 in osteosarcoma progression and its underlying mechanisms.

Main Methods:

  • Utilized GTEx and TARGET databases to identify prognostic genes in osteosarcoma.
  • Quantified FKBP11 expression using qRT-PCR, Western blotting, and immunohistochemistry.
  • Assessed the functional role of FKBP11 in osteosarcoma cells via CCK-8, Transwell, colony formation, and flow cytometry assays.

Main Results:

  • FKBP11 exhibited high expression in osteosarcoma tissues.
  • Silencing FKBP11 suppressed osteosarcoma cell invasion, migration, proliferation, and induced apoptosis.
  • FKBP11 knockdown inhibited MEK/ERK phosphorylation, implicating the MAPK pathway.

Conclusions:

  • FKBP11 is a validated prognostic factor closely associated with osteosarcoma.
  • FKBP11 promotes malignant properties of osteosarcoma cells via the MAPK pathway.
  • This study offers a new therapeutic strategy targeting FKBP11 for osteosarcoma treatment.
Keywords:
FKBP11MAPK signaling pathwayosteosarcomaprognosis

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