Spiribacter salilacus sp. nov., a novel moderately halophilic bacterium isolated from a saline lake in China

A Gram-staining-negative, non-motile, rod-shaped or curved rod-shaped, moderately halophilic bacterium, designated C176^T, was isolated from Yuncheng Salt Lake in Shanxi Province, P.R. China. The optimal temperature, salinity and pH for growth of strain C176^T was 37 °C, 6% (w/v) NaCl and 7.5. Phylogenetic analysis using 16S rRNA gene sequences indicated strain C176^T has the highest similarity with Spiribacter salinus LMG 27464 ^T (97.7%), following by the S. halobius E85^T (97.6%), S. curvatus DSM 28542 ^T (97.2%), S. roseus CECT 9117 ^T (97.0%) and S. vilamensis DSM 21056 ^T (96.9%). The ANI and dDDH values between strain C176^T and S. salinus LMG 27464 ^T were 69.8 and 17.7%, respectively. The DNA G + C content of genome for strain C176^T was 54.1%. Summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c) and C_16:0 were detected as its major fatty acids, with content of 38.7 and 28.6% respectively, while Q-8 was the predominant ubiquinone. The major polar lipids of strain C176^T contained phospholipid, phosphatidylglycerol and phosphoglycolipid. In accordance with results of polyphasic taxonomy, strain C176^T is considered as a novel species of the genus Spiribacter, for which the name Spiribacter salilacus sp. nov. is proposed. The type strain is C176^T (= MCCC 1H00417^T = KCTC 72692 ^T).