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Related Experiment Video

Updated: Aug 4, 2025

A Method to Study de novo Formation of Chromatin Domains
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SENP3 and USP7 regulate Polycomb-rixosome interactions and silencing functions.

Haining Zhou1, Wenzhi Feng2, Juntao Yu2

  • 1Howard Hughes Medical Institute, Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA, USA; National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Cell Reports
|April 4, 2023
PubMed
Summary
This summary is machine-generated.

The deSUMOylating enzyme SENP3 and deubiquitinating enzyme USP7 are crucial for Polycomb silencing. These enzymes regulate the assembly and function of the rixosome and PRC1 complexes.

Keywords:
CP: Molecular biologySENP3SUMOUSP7deSUMOylationdeubiquitinationepigeneticsgene silencingheterochromatinpolycombrixosomeubiquitin

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • The rixosome and Polycomb Repressive Complex 1 (PRC1) are involved in gene silencing.
  • The roles of deSUMOylation and deubiquitination in these silencing pathways are not fully understood.

Purpose of the Study:

  • To investigate the requirement of SENP3 and USP7 enzymatic activities in Polycomb-mediated gene silencing.
  • To elucidate how deSUMOylation and deubiquitination regulate the rixosome and PRC1 complexes.

Main Methods:

  • Enzymatic activity assays for SENP3 and USP7.
  • Co-immunoprecipitation to study complex formation.
  • Western blotting to detect protein modifications and complex assembly.
  • Reporter gene assays to assess gene silencing.

Main Results:

  • SENP3 and USP7 enzymatic activities are essential for silencing Polycomb target genes.
  • SENP3 deSUMOylates rixosome subunits, facilitating its association with PRC1.
  • USP7 deubiquitinates PRC1 subunits (CBX2, CBX4), and its inhibition leads to PRC1 disassembly.
  • Both SENP3 and USP7 are required for silencing at an ectopic reporter locus.

Conclusions:

  • SUMOylation and ubiquitination dynamically regulate the assembly and activity of the rixosome and PRC1 complexes.
  • These post-translational modifications likely play critical roles in developmental processes and cellular responses to environmental changes.