Long-term preservation and genetic stability of entomopathogenic fungal species
Miguel Angel Ayala-Zermeño1, Angélica María Berlanga-Padilla1, Carlos Fernando Regla-Márquez1
1Departamento de Control Biológico, CNRF-DGSV-SENASICA-SADER. Km 1.5 Carretera Tecomán-Estación FFCC, Col. Tepeyac C.P. 28110, Tecomán, Colima, Mexico.
Abstract:
Entomopathogenic fungi (EPF) preservation aims to maintain valuable characteristics. Growth, conidiation and genetic stability of eight species of EPF were evaluated in six preservation methods for up to 8.2 years. Cryopreservation at -196 °C, freeze-drying, and ultra-freezing at -70 °C resulted as the best methods for long-term storage.
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